静电纺丝法制备小口径胶原-聚乳酸人工血管

以聚乳酸(PLA)和胶原(Col)为原料,通过静电纺丝法制备了小口径(d=3.0mm)Col-PLA人工血管。采用扫描电镜、孔径分析仪和万能拉力机对Col-PLA人工血管的外层纤维形貌、孔径和拉伸性能进行了表征,测定了人工血管的管壁厚度及爆破强度。研究了纺丝电压、纺丝液质量分数、PLA与Col质量比对人工血管结构及性能的影响。结果表明:随纺丝电压的增加,纤维排列由杂乱变为规整,最佳的纺丝电压为15~20kV之间;当纺丝液质量分数增大时,Col-PLA人工血管的纤维直径增大,孔径及孔隙率均变小,拉伸强度和爆破强度提高;随PLA与Col质量比提高,人工血管的内层管壁厚度减少,外层管壁厚度增加,使拉伸强度和爆破强度提高,PLA与Col质量比分别为70∶30和90∶10时,制得人工血管力学强度能够满足使用要求。

Preparation of small-diameter collagen-polylactic acid artificial blood vessel by electrospinning

The small diameter artificial blood vessel(d=3.0 mm)of polylactide acid and collagen (Col-PLA)was prepared by electrospinning in this paper.The fiber morphology on the surface,pore size and tensile property of Col-PLA artificial blood vessel were characterized by scanning electron microscopy,pore size analyzer and tensile testing machine,respectively.The wall thickness and burst strength were also measured.The influence of spinning voltage,spinning solution mass fraction and mass ratio of PLA to Col on the structure and properties of artificial blood vessel were investigated.The results show that the fiber array is changed into regular with the increasing of the spinning voltage,and the optimal spinning voltage is between 15-20 kV.The fiber diameter and the mechanical properties of artificial blood vessel increase with the increasing of the spinning solution mass fraction,while the po-rosity and pore size decrease.As mass ratio of PLA to Col increase,the inner layer thickness of Col-PLA artificial blood vessel decrease and the outer layer thickness increase,which lead to the tensile strength and burst strength of the artificial blood vessel improvement.When the mass ratio of PLA to Col are 70:30 and 90:10,the mechanical properties of artificial vessel can meet the requirements.