| 放线菌发酵提取物对百合炭疽病的抗菌活性筛选 |
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| 引用本文： | 杨佩文,李艳琼,尚慧,李铭刚,曾莉,李一青,刘树芳,崔晓龙,李家瑞,文孟良. 放线菌发酵提取物对百合炭疽病的抗菌活性筛选[J]. 农药, 2006, 45(8): 571-573 |
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| 作者姓名： | 杨佩文 李艳琼 尚慧 李铭刚 曾莉 李一青 刘树芳 崔晓龙 李家瑞 文孟良 |
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| 作者单位： | 云南省农业科学院,农业环境资源研究所,昆明,650205;云南大学,教育部微生物资源开放研究重点实验室,云南省生物资源保护与利用重点实验室,昆明,650091 |
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| 摘 要： | 从云南不同生态环境中采集的土壤样品巾分离到334株放线菌，以百合炭疽病菌作为指示菌，通过菌株发酵提取物对孢子萌发抑制、菌丝生长抑制和温室盆栽防治效果的测定，初步筛选出对炭疽病菌分生孢子萌发有较强抑制能力的18个菌株，对炭疽病菌菌丝生长有较强抑制能力的24个菌株，离体叶片防治效果60％以上的14个菌株，其中YIM31927、YIM31047等10株发酵提取物对百合炭疽病菌孢子萌发、菌丝生长和离体叶片防治试验均表现出了一致活性，筛选到一批具有开发应用潜能的放线菌资源。
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| 关 键 词： | 百合炭疽病 放线菌发酵提取物 抗菌活性 |
| 文章编号： | 1006-0413（2006）08-0571-03 |
| 收稿时间： | 2005-12-03 |
| 修稿时间： | 2005-12-032006-04-10 |
Screening actinomycete fermentation extracts for inhibitory activity against Colletotrichum liliacearum |
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| YANG Pei-wen,LI Yan-qiong,SHANG Hui,LI Ming-gang,ZENG Li,LI Yi-qing,LIU Shu-fang,CUI Xiao-long,LI Jia-rui,WEN Meng-liang. Screening actinomycete fermentation extracts for inhibitory activity against Colletotrichum liliacearum[J]. Pesticides, 2006, 45(8): 571-573 |
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| Authors: | YANG Pei-wen LI Yan-qiong SHANG Hui LI Ming-gang ZENG Li LI Yi-qing LIU Shu-fang CUI Xiao-long LI Jia-rui WEN Meng-liang |
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| Affiliation: | 1.lnstitute of Agriculture Environment and Resource, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; 2.Key Laboratory for Microbial Resources of Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China |
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| Abstract: | Three hundred thirty four actinomycete strains were isolated from soil samples taken from diverse ecologi-cal environments in Yunnan Province. Using Colletrotrichum liliacearum as the indicator species, greenhouse potexperiments were conducted to determine efficacy of fermentation extracts from the actinomycetes in inhibiting sporegermination and mycelial growth. Preliminary screening identified 18 strains with strong inhibition of conidia germination,24 strains that inhibited mycelial growth, and 14 strains showing >60% curative activity on detached leaves. Amongthese, fermentation extracts from 10 strains, including YIM31927 and YIM31047, provided consistent Colletrotrichumliliacearum control as determined by spore germination inhibition, mycelial growth inhibition, and curative effects ondetached leaves. These actinomycete strains have potential for development as fungicides. |
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| Keywords: | Colletotrichum liliacearum actinomycete fermentation extracts fungal inhibition activity |
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