Improved reutilization of industrial crude lysine to 1,5-diaminopentane by enzymatic decarboxylation using various detergents and organic solvents |
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| Authors: | Hanyong Kim Hah Young Yoo Yong Hwan Ki Il-Kwon Kim Eui-Hong Byun Yung-Hun Yang Si Jae Park Jeong-Geol Na Hiesang Sohn Taek Lee Jung Rae Kim Chulhwan Park |
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| Affiliation: | 1.Department of Chemical Engineering,Kwangwoon University,Seoul,Korea;2.Department of Biotechnology,Sangmyung University,Seoul,Korea;3.School of Energy and Chemical Engineering,Ulsan National Institute of Science and Technology (UNIST),Ulsan,Korea;4.Department of Bioprocess Research,Daesang Co. Ltd.,Gyeonggi,Korea;5.Department of Food Science and Technology,Kongju National University,Chungnam,Korea;6.Department of Biological Engineering,Konkuk University,Seoul,Korea;7.Division of Chemical Engineering and Materials Science,Ewha Womans University,Seoul,Korea;8.Department of Chemical and Biomolecular Engineering,Sogang University,Seoul,Korea;9.School of Chemical and Biomolecular Engineering,Pusan National University,Busan,Korea |
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| Abstract: | World-wide production of l-lysine has rapidly increased in recent years. In the industrial scale production, it is cost effective to minimize waste as many waste materials are generated during downstream processing. Therefore, the conversion of crude lysine to a more valuable product reduces waste emission. In this study, 1,5-diaminopentane (DAP, trivial name: cadaverine) was produced by l-lysine decarboxylation using Hafnia alvei. The conditions of enzymatic reaction were determined. In particular, the addition of specific detergent (Brij 56) was significantly affected in the bioconversion system. Addition of hydrophobic organic solvent improved the mixing of the reactants. Finally, an industrial crude form of lysine served as a substrate. The DAP conversion by analytical, feed and industrial crude l-lysine was 93.9%, 90.3%, and 63.8%, respectively. |
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