Chemoenzymatic synthesis of amylose-grafted polyacetylenes

Summary This paper reports chemoenzymatic synthesis of amylose-grafted polyacetylenes according to the following reaction manners. Polymerization of a N-propargylamide monomer having a maltooligosacchairde chain was firstly carried out using a water-soluble Rh-catalyst in water, giving maltooligosaccharide-grafted polyacetylene. The ¹H NMR spectrum of the product supported the structure of the desired polyacetylene. Then, the enzymatic chain-elongation from the oligosaccharides of the polymer was performed using glucose-1-phosphate catalyzed by phosphorylase to give the polyacetylene having amylose side-chains. Furthermore, copolymerization of the monomer with the other N-propargylamide monomer was performed under the conditions similar to those of homopolymerization. The phosphorylase-catalyzed enzymatic chain-elongation of the copolymer was also carried out. The DLS measurement of the amylose-grafted polyacetylenes in alkaline solution was conducted.