高效液相色谱法同时测定食品中10种合成着色剂

目的 建立一种高效液相色谱法同时测定食品中10种合成着色剂(柠檬黄、新红、觅菜红、靛蓝、胭脂红、日落黄、诱惑红、亮蓝、喹琳黄、赤藓红).方法 用GB/T 5009.35-2003(食品中人工合成着色剂的测定方法)中试样处理方法制备样品溶液,聚酰胺吸附法提取色素,以甲酵/乙睛(3+1)+乙酸铵(0.02 mol/L,pH =4.0)为流动相,梯度淋洗分离,多波长检测定量.结果 线性范围分别为柠檬黄、觅菜红、靛蓝、胭脂红、日落黄于0.94-30.00p.g/ml,新红于0.94～30.00μg/ml,诱惑红于1.00～32.10μg/ml,亮蓝于0.31～10.00μg/ml,喹琳黄于1.219.68μg/ml,赤藓红于0.76～24.40μg/ml,线性关系良好,相关系数0.999 0～1.000 0,回收率87.5% - 101.4%,RSD 1.3%～5.7%,检出限分别为0.03～ 0.9 mg/kg.结论 该方法简单、准确、灵敏,适用于食品中10种合成着色剂的定量分析.

Simultaneous determination of 10 synthetic colorants in foods with high performance liquid chromatography

Objective To establish a simple,sensitive and accurate method for the determination of ten kinds of synthetic colorant in food at the same time.Methods Synthetic colorants,including Tartrazine,New Red,Amaranth,Indigo Blue,Ponceau 4R,Sunset Yellow,Allura Red,Brillinat Blue,Quinoline Yellow and Erythrosine B in food samples were prepared with the procedures described in GB/T 5009.35 and were extracted by polyamide adsorption.Sample solutions were separated by reverse phase liquid chromatography with carbinol/...