New hydrogels based on maleilated collagen with potential applications in tissue engineering |
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| Authors: | Simona PotoracMarcel Popa Vasilica MaierGabriela Lisa Liliana Verestiuc |
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| Affiliation: | a “Gheorghe Asachi” Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Natural and Synthetic Polymers, 71 Dimitrie Mangeron, 700050 Iasi, Romaniab “Gheorghe Asachi” Technical University, Faculty of Textile, Leather and Industrial Management, Department of Chemical Technology of Leather and Substitutes, 71 Dimitrie Mangeron, 700050, Iasi, Romaniac “Gr.T.Popa” University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biological Sciences, 9-13 Kogalniceanu Street, 700454, Iasi, Romania |
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| Abstract: | New hydrogels based on maleic anhydride (MA) modified collagen were prepared with the aim of overcoming the high degradation rate displayed by collagen that is not otherwise chemically crosslinked. Semi-interpenetrated matrices were obtained by free radical polymerization of maleilated collagen (CM) and 2-hydroxyethyl methacrylate (HEMA) in the presence of ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED) as initiating system. The resulting matrices (CMH) had a sharp decrease in degradation, when compared to pure collagen. FTIR and H1 NMR spectroscopies were used to confirm the incorporation of MA on the collagen peptide chains. The final composition of CMH was found to be strongly dependent by the concentration of maleilated collagen. The morphology of the hydrogels was studied by Scanning electron microscopy (SEM) and the macro-gel structure was confirmed. Water uptake of the synthetised hydrogels is influenced by both composition and the porosity of the matrices. |
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| Keywords: | C, native collagen MA, maleic anhydride CM, maleilated collagen CMH, hydrogels based on maleilated collagen and HEMA HEMA, 2-hydroxy ethyl methacrylate pHEMA, poly(2-hydroxy ethyl methacrylate) APS, ammonium persulfate TEMED, N,N,N&prime ,N&prime -tetramethylethylenediamine DMSO, dimethyl sulfoxide 15.8CM2APS, collagen hydrogel with CM:HEMA = 15.8:84.2 and 2% APS (APS:HEMA %mol:mol) 44.1CM2APS, collagen hydrogel with CM:HEMA = 44.1:55.9 and 2% APS (APS:HEMA %mol:mol) 30CM2APS, collagen hydrogel with CM:HEMA = 30:70 and 2% APS (APS:HEMA %mol:mol) SIPN, semi-interpenetrating polymer network PBS, phosphate buffered saline TMS, tetramethylsilane WU, water uptake wt, weight at time point t w0, initial sample weight DD, degradation degree Nh, concentration of α-NH2 groups (mmol/ml × 10&minus 10) for the obtained hydrogels NC, concentration of α-NH2 groups (mmol/ml × 10&minus 10) for pure collagen a0, regression coefficient xi xj, input variables Y, response variable COR, correlation coefficients ASE, assymetric errors |
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