Hydrogen production from glucose by co-culture of Clostridium Butyricum and immobilized Rhodopseudomonas faecalis RLD-53 |
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Authors: | Jie Ding Bing-Feng Liu Nan-Qi Ren De-Feng Xing Wan-Qian Guo Ji-Fei Xu Guo-Jun Xie |
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Affiliation: | State Key Lab of Urban Water Resource and Environment (SKLUWRE), School of Municipal and Environmental Engineering, Harbin Institute of Technology, No. 202 Haihe Road, 2nd Campus of HIT Box 2614, Harbin 150090, China |
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Abstract: | In this work, the effects of different parameters on co-culture hydrogen production using Clostridium Butyricum and immobilized Rhodopseudomonas faecalis RLD-53 were investigated. The maximum hydrogen yield of 4.134 mol H2/mol glucose was obtained using 6 g/l glucose, 50 mmol/l phosphate buffer, initial pH of 7.5, the ratio of dark to photo bacteria of 1:10 and light intensity of 8000 lux. The maximum hydrogen production rate was 33.85 ml H2/l/h. Phosphate buffer concentration was the most important parameter influencing hydrogen production in this co-culture. The ratio of acetate to butyrate increased from 0.74 to 1.82 in the soluble metabolites from C. butyricum with phosphate buffer concentration of 10–50 mmol/l. Experimental results could be of great significance for further pilot studies of co-culture hydrogen production. |
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