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| 红曲霉中PKS基因的结构分析 | |
| 引用本文： | 丘振宇,曾军,林勇,杨宇,王亚琴. 红曲霉中PKS基因的结构分析[J]. 酿酒科技, 2010, 0(4): 17-20 |
| 作者姓名： | 丘振宇 曾军 林勇 杨宇 王亚琴 |
| 作者单位： | 1. 贵州省仁怀市茅台镇老伙记酒业有限公司,贵州,仁怀,564501;华南理工大学生物科学与工程学院,广东,广州,510640 2. 贵州省仁怀市茅台镇老伙记酒业有限公司,贵州,仁怀,564501 3. 华南理工大学生物科学与工程学院,广东,广州,510640 |
| 基金项目： | 863国家基金资助项目(024-D90661);;广东省自然科学基金资助项目(07006553) |
| 摘 要： | 对实验室保藏的一株高产Monacolin K红曲霉菌株及其野生株进行了初步的PKS基因结构分析。扩增得到红曲霉野生株W菌及其诱变株M菌的目的基因序列,并将测序产物和cDNA序列进行比对,确定该段基因序列不含内含子,且诱变株M菌的突变位置不在此段基因上。分别用ProfileScan、SWISS-MODEL预测了该段蛋白的结构域以及三维结构。 |
| 关 键 词： | 微生物 红曲霉 PKS基因 结构研究 |
Analysis of PKS Gene Structure of Monascus |
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| QIU Zhen-yu,ZENG Jun,LIN Yong,YANG Yu,WANG Ya-qin. Analysis of PKS Gene Structure of Monascus[J]. Liquor-making Science & Technology, 2010, 0(4): 17-20 | |
| Authors: | QIU Zhen-yu ZENG Jun LIN Yong YANG Yu WANG Ya-qin |
| Affiliation: | 1.Laohuoji Liquor Industry Co.Ltd./a>;Renhuai/a>;Guizhou 564501/a>;2.School of Bioscience and Bioengineering/a>;South China University of Technology/a>;Guangzhou/a>;Guangdong 510641/a>;China |
| Abstract: | The PKS Gene structure of a lab-preserved Monascus strain with high yield of Monacolin K and its wild strain was analyzed.Then the gene sequence of Monascus wild strain W and its mutant strain M were amplified by polymerase chain reaction(PCR).And the sequencing products were compared with cDNA sequence.The results confirmed that such gene segment had no intron and the mutation position of mutant strain M did not locate on such gene segment.Finally,the domain and 3D structure of the protein in such segment ... |
| Keywords: | microbe Monascus PKS Gene structure analysis |
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