蔗渣酶法水解在非缓冲溶剂体系中的反应机理探讨

通过测定蔗渣酶法水解在水系介质中的反应过程的pH随各种反应条件的变化，探讨了酶解液的各种特性，并测定了甘蔗渣、纤维素及纤维素酶相互作用的红外图谱。通过分析认为，纤维素在酶水解过程中，纤维素和纤维素酶相互作用形成了中间产物，使纤维素酶的立体构象发生变化而放出H^ ，使酶解液的pH发生变化，并形成了一种微弱的缓冲体系，致使甘蔗渣酶解反应在采用普通纯水代替缓冲液时，酶解反应的还原糖得率不变。

Study on the Mechanism of Bagasse Enzymatic Hydrolysis in Water Media

In this paper, the pH changes with many conditions in the water media of bagasse enzymatic hydrolysis were studied. And the reaction between hydrolyzate and dilute NaOH was studied. The infrared chart of bagasse, cellulose and enzyme were analyzed. The pH of the hydrolyzate was in the range of 4.5~5.0, which coincided with the optimum pH for the enzyme reaction. It was considered that the enzyme combined with the substrate and formed a transition complex compound in hydrolysis process. Then the configuration of the enzyme was changed and H + was released. The transition complex compound formed a buffer system. It made the pH maintain around 5.0 and it did not decrease further in the enzymatic hydrolysis process. The results indicated that industrialization of the enzymatic hydrolysis in a water media is feasible.