Immobilization of horseradish peroxidase on self-assembled (3-mercaptopropyl)trimethoxysilane film: Characterization, direct electrochemistry, redox thermodynamics and biosensing |
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Authors: | Fanghua Wu Zhichao Hu Jingjing Xu Yuan Tian Liwei Wang Yuezhong Xian Litong Jin |
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Affiliation: | aDepartment of Chemistry, East China Normal University, Shanghai 200062, China;bJiangsu Key Laboratory for Chemistry of Low-Dimensional Materials (Huaiyin Teachers College), Huaian 223300, China |
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Abstract: | Highly organized (3-mercaptopropyl)trimethoxysilane (3-MPT) films have been prepared via self-assembled coupled with sol–gel linking technology. Horseradish peroxidase (HRP) is successfully immobilized onto the densely packed three-dimensional (3D) 3-MPT network and the direct electrochemistry of HRP is achieved without any electron mediators or promoters. Redox thermodynamics of HRP on the 3-MPT films, which is obtained from the temperature dependence of the reduction potential, suggests that the positive shift of redox potentials of HRP at the interface of 3-MPT originates from the solvent reorganization effects and conformational change of the polypeptide chain of HRP. Based on the direct electrochemistry and electrocatalytic ability of HRP, a sensitive third-generation amperometric H2O2 biosensor is developed with two linear dependence ranges of 5.0 × 10−7 to 1.0 × 10−4 and 1.0 × 10−4 to 2.0 × 10−2 mol L−1. |
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Keywords: | Horseradish peroxidase (3-mercaptopropyl)trimethoxysilane Self-assemble Direct electrochemistry Redox thermodynamics H2O2 biosensor |
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