细菌纤维素/明胶复合多孔支架材料的制备与表征

介绍了以细菌纤维素水凝胶膜和明胶为原料制备细菌纤维素/明胶多孔复合支架的方法,并利用红外光谱、扫描电子显微镜、X射线衍射及力学性能测试对多孔复合支架进行研究。结果表明,复合多孔支架的表面孔径变大、孔隙率下降,但依然呈三维网络结构;明胶的加入使细菌纤维素的链规整度下降,结晶度变小、力学性能下降;同时,明胶能够调节细胞的响应并且促进细胞的贴附和生长,使细菌纤维素/明胶复合多孔支架更有利于细胞的粘附、增殖,更适用于生物医学领域。

Preparation and Characterization of Bacterial Cellulose/Gelatin Composite Porous Scaffold

In this research,a bacterial cellulos(BC)/gelatin composite porous scaffold has been successfully prepared by using BC gel and gelatin solution as raw materials.Characteristics of this BC/gelatin composite porous scaffold are investigated by Fourier transform infrared spectrum(FT-IR),scanning electron microscopy(SEM),X-ray diffraction(XRD) and the tensile test.The results show that the composite scaffold has very well interconnected porous network structure and large aspect surface.Gelatin molecules are not only coated on the BC fibrils surface but also penetrate inside BC.With the introduction of gelatin,the crystal structure of BC becomes less regular,crystallinity and tensile properties tend to decrease,which might be associated with strong interaction between BC and gelatin.The cells incubated in BC/gelatin composite porous scaffold for 48 h are capable of forming cell adhesion and proliferation.It shows much better biocompatibility than pure BC.Therefore,this BC/gelatin scaffold is more suitable for biomedical applications.