| 系统Ⅱ乙烯对采后番茄果实抗冷过程中抗氧化酶的影响 |
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| 引用本文： | 吕胜男,赵瑞瑞,申琳,赵丹莹,生吉萍. 系统Ⅱ乙烯对采后番茄果实抗冷过程中抗氧化酶的影响[J]. 食品科学, 2012, 33(8): 296-300. DOI: 10.7506/spkx1002-6630-201208065 |
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| 作者姓名： | 吕胜男 赵瑞瑞 申琳 赵丹莹 生吉萍 |
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| 作者单位： | 1.中国农业大学食品科学与营养工程学院2.中国人民大学农业与农村发展学院3.北京市西城区疾病预防控制中心 |
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| 基金项目： | 国家自然科学基金面上项目(31071623;30972065);国家公益性行业(农业)科研专项(200803033) |
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| 摘 要： | 以反义ACS2 番茄、野生型丽春果实和经乙烯利处理的反义ACS2 番茄果实分别作为系统Ⅱ乙烯缺失模型、系统Ⅱ乙烯正常合成模型和系统Ⅱ乙烯补偿模型，研究系统Ⅱ乙烯对低温胁迫下氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的影响。研究发现，丽春果实和经乙烯利处理的反义ACS2果实较未处理的反义ACS2果实组冷害指数低，电解质渗透率的增长被抑制，CAT、APX、SOD平均活性水平提高，而两组的POD活性增长分别被抑制和诱导。这说明系统Ⅱ乙烯能提高CAT、APX、SOD活性水平并调控POD活性变化，对增强低温胁迫下番茄果实的抗冷能力有积极作用。
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| 关 键 词： | 乙烯 抗氧化酶 氧化胁迫 抗冷性 番茄 |
Effect of System-2 Ethylene on Antioxidant Enzyme Activities in Cold Resistance Process of Tomato Fruits |
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| LU..Sheng-nan,ZHAO Rui-rui,SHEN Lin,ZHAO Dan-ying,SHENG Ji-ping. Effect of System-2 Ethylene on Antioxidant Enzyme Activities in Cold Resistance Process of Tomato Fruits[J]. Food Science, 2012, 33(8): 296-300. DOI: 10.7506/spkx1002-6630-201208065 |
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| Authors: | LU..Sheng-nan ZHAO Rui-rui SHEN Lin ZHAO Dan-ying SHENG Ji-ping |
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| Affiliation: | (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；2. School of Agricultural Economics and Rural Development, Renmin University of China, Beijing 100872, China；3. Beijing Xicheng District Center for Disease Control and Prevention, Beijing 100011, China) |
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| Abstract: | To analyze the effect of system-2 ethylene on the activities of catalase(CAT),ascorbate peroxidase(APX),peroxidase(POD) and superoxide dismutase(SOD) during exposure to cold stress,anti-sense ACS2,wild-type Lichun and ethephon-treated anti-sense ACS2 tomato fruits were used to the models of non-system-2 ethylene synthesis,normal system-2 ethylene synthesis and compensative system-2 ethylene synthesis,respectively.It was indicated that wild-type Lichun and ethephon-treated anti-sense ACS2 tomato fruits decreased chilling injury index,inhibited the increase of electrolyte leakage and increased the average activities of CAT,APX and SOD compared with native anti-sense ACS2 tomato fruits.The increase of POD activity was inhibited in wild-type Lichun but induced in ethephon-treated anti-sense ACS2.From these results,it can be concluded that system-2 ethylene can improve the cold resistance of tomato during exposure to cold stress by increasing CAT,APX and SOD activities and regulating POD activity. |
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| Keywords: | ethylene antioxidant enzymes oxidative stress cold resistance tomato |
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