Improved multiplex PCR assay for simultaneous detection of Bacillus cereus emetic and enterotoxic strains |
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| Authors: | Jae-Myung Kim Fereidoun Forghani Jung-Beom Kim Yong-Bae Park Myoung-Su Park Jun Wang Joong Hyun Park Deog-Hwan Oh |
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| Affiliation: | 1. Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea
2. Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment, Suwon, Gyeonggi, 440-290, Korea
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| Abstract: | Toxin producing Bacillus cereus can cause enterotoxic and/or emetic food poisoning. In the present study, a multiplex PCR assay was developed to detect all toxin genes known to be involved in food poisoning of B. cereus in a single reaction. Specific primers for the detection of enterotoxic (entFM, hblC, nheA, and cytK) genes and emetic toxin production (2 primer pairs: ces, CER) were designed based on the GeneBank sequences. The developed multiplex PCR assay was evaluated in pure culture and artificially inoculated milk, using 43 B. cereus strains and non-target strains. In brief, sensitivity in pure culture was 10-fold or more higher than artificially inoculated milk in multiplex PCR detection limit assay. The presented PCR assay is a developed molecular tool for the rapid simultaneous detection of emetic and enterotoxin producing B. cereus strains. |
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