Extraction of pure lycopene from industrial tomato by‐products in water using a new high‐pressure process |
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Authors: | Daniele Naviglio Fabiana Pizzolongo Lydia Ferrara Alejandro Aragòn Antonello Santini |
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Affiliation: | 1. Dipartimento di Scienza degli Alimenti, Università di Napoli Federico II, Via Università 100, 80055 Portici (Napoli), Italy;2. Dipartimento di Chimica Farmaceutica e Tossicologia, Università di Napoli Federico II, Via Montesano 49, 80131 Napoli, Italy;3. Facultad de Ciencias Agrarias y Forestales, Universidad de La Plata, Calle 60 y 119, 1900 La Plata (Buenos Aires), Argentina |
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Abstract: | BACKGROUND: Lycopene, a precursor of β‐carotene with a well‐known antioxidant activity, contained in many natural products such as tomato (Lycopersicon esculentum Mill.), watermelon, red pepper and papaya, is usually recovered from natural vegetal sources using organic solvents and a purification step. In this paper an innovative process for the extraction of pure lycopene from tomato waste in water that uses the Naviglio® extractor and water as extracting phase is presented. RESULTS: Lycopene was obtained in the all‐trans form at a very high grade of purity, not less than 98% (w/w), with an average recovery of 14% (w/w). The availability of high‐purity trans‐lycopene allowed measurement of the molar absorption coefficient. An alternative procedure for high‐performance liquid chromatographic analysis using a phenyl‐hexyl silicone phase as inverse phase and a linear gradient in water and acetonitrile is also described. CONCLUSIONS: The use of water as extracting phase considerably reduces the cost of the entire process when compared with the commonly used solvent‐based procedure or with the newer supercritical extraction process of lycopene from tomato waste. Lycopene, not soluble in water, was recovered in a quasi‐crystalline solid form and purified by solid‐phase extraction using a small amount of organic solvent. Copyright © 2008 Society of Chemical Industry This article was published online on September 15, 2008. Errors in Figures 2 ‐ 4 were subsequently identified. The publishers wish to apologise for these errors. This notice is included in the online and print versions to indicate that both have been corrected [September 19, 2008] |
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Keywords: | lycopene Naviglio® extractor tomato waste HPLC diode array solid– liquid extraction solid‐phase extraction NMR |
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