| 不同水解方式下蚕丝丝素蛋白材料的止血性能 |
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| 引用本文： | 雷彩虹,俞林双,朱海霖,郑涛,陈建勇.不同水解方式下蚕丝丝素蛋白材料的止血性能[J].纺织学报,2022,43(4):15-19. |
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| 作者姓名： | 雷彩虹 俞林双 朱海霖 郑涛 陈建勇 |
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| 作者单位： | 1.浙江理工大学 纺织科学与工程学院(国际丝绸学院), 浙江 杭州 3100182.浙江省纤维材料和加工技术研究重点实验室, 浙江 杭州 310018 |
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| 基金项目： | 浙江省基础公益研究计划项目(LGC21E030003);浙江省教育厅一般科研项目(20200066-F);国家自然科学基金项目(51273181);浙江理工大学科研启动项目(19202448-Y) |
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| 摘 要： | 为提升蚕丝丝素蛋白材料的止血性能,采用盐解和酶解2种方式水解蚕丝丝素蛋白,通过冷冻干燥法制备得到2种蚕丝丝素蛋白材料.通过凝胶过滤色谱、水溶性实验、凝血因子实验、血小板黏附实验、酶联免疫吸附实验等分析了2种蚕丝丝素蛋白材料的分子质量、水溶性和止血效果.结果表明:与盐解法相比,酶解所得材料具有较低的分子质量和较好的水溶性...
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| 关 键 词： | 蚕丝 丝素蛋白 水解方式 盐解 酶解 止血性能 |
| 收稿时间： | 2021-03-05 |
Hemostasis properties of silk fibroin materials under different types of hydrolysis |
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| LEI Caihong,YU Linshuang,ZHU Hailin,ZHENG Tao,CHEN Jianyong.Hemostasis properties of silk fibroin materials under different types of hydrolysis[J].Journal of Textile Research,2022,43(4):15-19. |
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| Authors: | LEI Caihong YU Linshuang ZHU Hailin ZHENG Tao CHEN Jianyong |
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| Affiliation: | 1. College of Textile Science and Engineering(International Institute of Silk), Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China2. Zhejiang Provincial Key Laboratory of Fiber Materials and Manufacturing Technology, Hangzhou, Zhejiang 310018, China |
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| Abstract: | Aiming at the improvement of hemostasis properties of silk fibroin materials fabricated by salt hydrolysis and enzyme hydrolysis method, the silk fibroin materials were prepared via a freeze-drying method. The molecular weight, water solubility and hemostatic effect of the resultant material were analyzed by gel filtration chromatography, water solubility test, coagulation factor test, platelet adhesion test, enzyme-linked immunosorbent assay and rat liver injury model. The results showed that compared with the salt hydrolysis method, the enzyme hydrolysis method could endow a lower molecular weight and better water soluble, which is conducive to activating the coagulation factor XII to promote endogenous coagulation. Moreover, the platelet adhesion and platelet factor 4 content increase significantly, the amount of bleeding from the rat liver is reduced with shortened bleeding time, indicating better hemostatic effect than that of Yunnan white medicine. |
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| Keywords: | silk silk fibroin types of hydrolysis salt hydrolysis enzyme hydrolysis hemostasis property |
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