超声波处理对兔毛角蛋白组成与结构的影响

针对化学试剂或高温处理促进二硫键断裂但导致酰胺键水解生成小分子多肽的问题,采用超声波辅助还原法提取了兔毛角蛋白,并使用蛋白质电泳仪、红外光谱仪、荧光光谱仪和粒度分析仪等研究了超声波处理时间对兔毛角蛋白的氨基酸组成和各级结构的影响.结果表明:随着超声波处理时间的增加,兔毛角蛋白的氨基酸组分没有发生变化,但氨基酸含量降低,...

Effect of ultrasonic treatment on composition and structure of rabbit hair keratin

In view of the destruction of disulfide bonds by increasing chemical reagents or high temperature treatment, resulting in the hydrolysis of amide bonds to generate small molecular polypeptides, ultrasonic treatment combined with reduction method was used to extract the keratin from the rabbit hair. The effects of ultrasonic treatment on the chemical composition and structure of rabbit hair keratin were investigated by protein electrophoresis meter, infrared spectrometer, fluorescence spectrometer and particle size analyzer. The results show that with the increase of ultrasonic treatment time, the amino acid composition of rabbit hair keratin remained constant, but the amino acid content decreased. The loss rate of cystine increased and the content of free sulfhydryl group firstly increases and then decreases, and the molecular weight increases gradually and mainly distribute in the range of 31-43 ku. The secondary structure changes from α-helix structure to β-sheet structure and random coil structure, and the particle size of the keratin decrease. The fluorescence spectra show that keratin has fixed structure and is not easy to agglomerate.