光合抑制法水质毒性检测中藻类培养光照与温度实验分析

浮游藻类作为单细胞生物，其光合活性外界胁迫作用响应灵敏，是水质综合毒性检测的良好受试生物，因此认识受试藻种光合活性状态对温度和光照等主要环境因子的响应规律，掌握有效控制受试藻种光合活性状态和浓度的培养条件，对水质综合毒性检测至关重要。以模式受试生物蛋白核小球藻为研究对象，研究了不同温度梯度和光照强度下蛋白核小球藻光合活性变化规律。研究结果显示：蛋白核小球藻在不同梯度光照下，藻种光合活性和藻种浓度的变化非常明显。低光照下(75μE、125μE)藻种平均光合活性在0.60左右但藻种浓度基本不增加；中光照下(175、225、300、375μE)藻种平均光合活性在0.57左右且此时藻种浓度有明显增加，其中，实验最佳光照为375μE；高光照下(475μE和600μE)藻种平均光合活性低于0.56 (初始活性)，会对藻种的生长状态产生胁迫。不同梯度温度下藻种光合活性和藻种浓度也有明显变化，中低温下(5、15、25℃)藻种平均光合活性在0.59左右且藻种浓度随着温度的升高而增加，最佳温度为25℃；高温下(30、35、40℃)藻种光合活性迅速下降直至失活。研究结果表明可以通过控制光照和温度来控制浮游藻...

Experimental analysis of illumination and temperature of algae culture in water toxicity detection by photosynthesis inhibition method

Planktonic algae are single-celled organisms, and their photosynthetic activity responds sensitively to external stress, so they are good test organisms for the comprehensive toxicity test of water quality. Obtaining the response law of the photosynthetic activity state of the tested algae species to the main environmental factors such as temperature and light, and mastering the culture conditions that effectively control the photosynthetic activity state and concentration of the tested algae species are essential for the comprehensive toxicity detection of water quality. Taking the model test organism Chlorella pyrenoidosa as the research object, the changes of photosynthetic activity of Chlorella pyrenoidosa under different temperature gradients and light intensities were studied in this work. The results show that the changes of photosynthetic activity and concentration of the algae species under different gradient illuminations are very obvious. Under low light (75 μE, 125 μE), the average photosynthetic activity of algae species is about 0.60 while the concentration of algae species basically does not increase. Under medium light (175, 225, 300, 375 μE), the average photosynthetic activity of algae species is around 0.57 and the concentration of algae species has increased significantly at this time. Among them, the best lighting for the experiment is 375 μE. Under high light (475 μE and 600 μE), the average photosynthetic activity of the algae species is lower than 0.56 (initial activity), which can stress the growth state of the algae species. It is also shown that the photosynthetic activity and concentration of algae species also changes significantly under different gradient temperatures. At medium and low temperatures (5, 15, 25 °C), the average photosynthetic activity of algae species is about 0.59, the concentration of algae species increases with the increase of temperature, and the best temperature is 25 ° C. Under high temperature (30, 35, 40 ° C), the photosynthetic activity of the algae species declines rapidly until inactivation. It is indicated that the photosynthetic activity and growth rate of planktonic algae can be controlled by controlling light and temperature, which can provide a standard method for cultivating the tested algae samples for a online water quality comprehensive toxicity meter, thereby laying a foundation for the development of the portable water quality comprehensive toxicity meter.