马铃薯酪氨酸酶的制备及其对羊毛的改性

为研究生物法对羊毛的改性，运用简易方法从马铃薯中提取酪氨酸酶，并以邻苯二酚（儿茶酚）为底物，研究自制马铃薯酪氨酸酶的活性和最佳的催化氧化条件，并用它处理羊毛织物。然后接枝含有－NH2的模拟化合物，并运用紫外和红外光谱进行分析,以此来检验酶处理羊毛是否发生氧化反应。结果表明：自制马铃薯酪氨酸酶催化产物的最大吸收波长为410 nm,最佳反应条件为pH值为6.8，温度30 ℃；羊毛织物的酪氨酸残基被催化氧化为多巴醌结构。

Preparation of potato-tyrosinase and modification of wool with it

In order to modify the wool with biological method, this paper investigated the activity and the best catalyzed oxidation conditions for preparation of Tyrosinase from potatoes using a simple and easy method with catechol (catecha phenol) as substrate. And the potato tyrosinase was used to treat the wool fabric. FTIR-ATR spectroscopy and the mimic complexes with－NH2 were used to examine whether the wool had occurrd oxidation reaction after enzymatic treatment. The results indicated that maximum absorbance wavelength of the self-made potato tyrosinase enzyme-induced reaction product is 410 nm with catechol as substrate, the best reaction condition is at 30 ℃ and pH 6.8. This research proved that tyrosine residue in wool was oxidized to dopa-quinone.