用吸收光谱和共振拉曼光谱研究水溶卟啉Cu（NACN）与DNA相互作用

本文用紫外一可见光谱和共振拉曼交谱发水溶金属卟啉４－Ｎ－乙腈基一吡啶基铜卟啉与ＤＮＡ的相互作用。分析表明，Ｃｕ（ＮＡＣＮ）以光沟槽方式与ＤＷＡ作用；与ＤＮＡ作用后，Ｃｕ（ＮＡＣＮ）的吡啶基团向垂直于卟啉环平面方向转过一个小的角度；在４４５ｎｍ的脉冲激光激发下，Ｃｕ（ＮＡＣＮ）与ＤＮＡ形成激发态复合物，而且Ｃｕ（ＮＡＣＮ）的激发态分子比基态分子现ＤＮＡ作用强。

Interaction between Water-soluble Porphyrin and DNA Studied by resonance Raman and UV-visible electronic spectra

The interaction of Water-soluble metalloporphyrin Cu(NACN) [Cu(4-N-acetonitrilepyridyl)porphine]With DNA was studied by UV-visible electronic and resonance Raman spectra.The analysis demonstrates: Cu(NACN) interacts with DNA by grove-binding; the pyridyl groups of Cu(NACN) were rotated a small angle toward perpendicular direction of porphyrin core Plane upon interaction with DNA;Cu(NACN) forms exciplex with DNA upon excited at 445nm Pulse; the interaction of excited Cu(NACN)with DNA is much more stronger than that of ground Porphyrin-DNA complex.