Application of modified pyrolytic graphite electrode as a sensor in the simultaneous assay of adenine and adenosine monophosphate |
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Authors: | Rajendra N. GoyalAuthor Vitae Sanghamitra ChatterjeeAuthor VitaeAnoop Raj Singh RanaAuthor Vitae Himanshu ChastaAuthor Vitae |
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Affiliation: | Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India |
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Abstract: | A simple and sensitive method based on square wave voltammetry (SWV) at single-walled carbon nanotube (SWNT) modified edge plane pyrolytic graphite electrode (EPPGE) is proposed for the simultaneous determination of adenine and adenosine-5′-monophosphate (5′-AMP). The modified electrode exhibits remarkable electrocatalytic properties towards adenine and 5′-AMP oxidation with a peak potential of ∼850 and 1165 mV respectively. Linear calibration curves are obtained over the concentration range of 5-100 nM for adenine and 10-100 nM for 5′-AMP with sensitivity of 677 and 476 nA nM−1 for adenine and 5′-AMP respectively. The limit of detection for adenine and 5′-AMP was found to be 37 × 10−10 M and 76 × 10−10 M, respectively. The effect of pH revealed that the oxidation of adenine and 5′-AMP at SWNT modified EPPGE involved equal number of electrons and protons. The modified electrode exhibited high stability and reproducibility. |
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Keywords: | Single-walled carbon nanotubes Adenine Adenosine-5&prime -monophosphate Square wave voltammetry Pyrolytic graphite electrode |
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