Functional reconstitution of protein ion channels into planar polymerizable phospholipid membranes |
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Authors: | Shenoy Devanand K Barger William R Singh Alok Panchal Rekha G Misakian Martin Stanford Vincent M Kasianowicz John J |
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Affiliation: | Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, USA. dshenoy@cbmse.nrl.navy.mil |
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Abstract: | We demonstrate that polymerizable planar membranes permit reconstitution of protein ion channels formed by the bacterial toxins Staphylococcus aureus alpha-hemolysin (alphaHL) and Bacillus anthracis protective antigen 63. The alphaHL channel remained functional even after membrane polymerization. Surface pressure measurements suggest that the ease of forming membranes depends on membrane surface elasticity estimated from Langmuir-Blodgett monolayer pressure-area isotherms. The ability to stabilize nanoscale pores in robust ultrathin films may prove useful in single molecule sensing applications. |
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