Modeling of protein adsorption to DEAE sepharose FF: Comparison of data with model simulation |
| |
Authors: | Mohammad Reza Aboudzadeh Zhu Jiawen Wu Bin |
| |
Affiliation: | (1) Nuclear Research Center for Agriculture & Medicine, AEOI, . Box 31585-4395, Karaj, Iran;(2) Chemical Engineering Research Center, East China University of Science and Technology, 200237 Shanghai, P.R. China |
| |
Abstract: | The equilibrium and kinetic characteristics of the adsorption of human serum albumin (HSA) and ovalbumin (OVA) to the DEAE
Sepharose FF weak anion exchanger were experimentally determined. The rate for protein adsorption was simulated with two different
models, the first being based on a single lumped kinetic parameter, while the second includes the individual mass transfer
processes occurring prior to the adsorption intervention, i.e., diffusion across the liquid film surrounding individual particles
and diffusion within the ion exchanger particle itself. The actual adsorption of OVA to DEAE Sepharose FF in fully mixed stirred
vessels and in packed bed columns was consistent with both models. In the case of HSA, however, the adsorption profile in
an agitated vessel was consistent only with the pore diffusion model and neither model could correctly predict the latter
part of the breakthrough profile observed in packed bed experiments. |
| |
Keywords: | Ion Exchange Chromatography Breakthrough Stirred Vessel Packed Bed Langmuir HSA OVA |
本文献已被 SpringerLink 等数据库收录! |
|