烟酰胺抑制人皮黑素细胞的适宜浓度研究

研究了烟酰胺对酪氨酸酶活性、人皮黑素细胞的增殖率、细胞周期动力学和黑素合成的影响。结果显示：烟酰胺体外抑制酪氨酸酶活性不明显；随浓度增加、作用时间的延长，烟酰胺抑制黑素细胞的增殖作用明显；0．25g／L烟酰胺加入24h后，细胞增殖指数明显下降，细胞多处于G0-G1期，S期细胞数量也显减少。实验发现：0．25g／L以上的烟酰胺均可以抑制黑素细胞中黑素合成，其中0．25g／L的烟酰胺可抑制黑素合成且对细胞损伤作用最小。故推荐0．25g／L的烟酰胺为安全、有效作用浓度。

Study of appropriate dose of nicotinamide to inhibit melanogenesis of human skin melanocytes in vitro

The impact of nicotinamide on the activity of tyrosinase,as well as the impact on the proliferation rate of human melanocytes,cell cycle kinetics and melanogenesis were studied.Results showed that:the inhibiting effects of nicotinamide on the activity of tyrosinase in vitro is not significant;Cell proliferation is significantly inhibited following the increasing of nicotinamide concentration and the lengthening of the contact time.With the nicotinamide dosage of 0.25 g/L and after 24 h,the proliferation index of melanocytes was decreased significantly and the cells are mainly in G_0-G_1 phase while the cells in S phase is significantly reduced.The discoveries obtained from the experiments show that 0.25 g/L is the appropriate nicotinamide concentration to inhibit the melanogenesis with the damage of the cells to be kept at a minimal.