Proteome changes in lamb semimembranosus muscles associated with the inclusion of sunflower cake in their diet |
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| Authors: | Aline Moreira Portela de Melo María López-Pedrouso Roberto Germano Costa Daniel Franco Arlindo de Alencar Araripe Noronha Moura Taciane Alves da Silva Frederico Bruno Mendes Batista Moreno Valdi de Lima Júnior Ana Cristina de Oliveira Monteiro-Moreira Ariosvaldo Nunes de Medeiros Renato de Azevedo Moreira Jose M Lorenzo |
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| Affiliation: | 1. Universidade Federal da Paraíba, 58937-000 Areia, Paraíba, Brazil;2. Department of Zoology, Genetics and Physical Anthropology, University of Santiago de Compostela, 15872 Santiago de Compostela, Spain;3. Centro Tecnológico de la Carne de Galicia, Parque Tecnológico de Galicia, Rúa Galicia No 4, 32900 Ourense, Spain;4. Universidade Federal do Ceará, 60020-181 Fortaleza, Ceará, Brazil |
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| Abstract: | A diet based on sunflower cake for lambs was assayed in order to reuse biodiesel industrial by-products with the aim of reducing livestock costs and evaluating their influence on meat quality. To achieve these goals, sixteen male lambs were fed diets containing different levels of sunflower cake (control, 5%, 10% and 15%). Afterwards, their semimembranosus muscles were analysed by two-dimensional electrophoresis coupled to mass spectrometry and their functional protein association was examined using STRING. Structural and metabolic proteins in the lambs’ proteomes changed significantly according to their diet. Fifteen proteins showed significant changes caused by the inclusion of sunflower cake, and the most differentially abundant structural proteins were detected in 2-DE gels from the lambs. Differentially abundant metabolic proteins such as ENO3 (enolase 3), MDH1 (malate dehydrogenase) and ALDH1A1 (retinal dehydrogenase) have been proposed as biomarkers of quality parameters in other species. |
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| Keywords: | ALDH1A1 biomarkers MDH1 metabolic proteins tenderness two-dimensional electrophoresis (2-DE) |
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