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Carotenoids and lipophilic antioxidant capacities of tomato purées as affected by high hydrostatic pressure processing
Authors:Maja Je?  Wioletta B?aszczak  Danuta Zielińska  Wies?aw Wiczkowski  Ireneusz Bia?obrzewski
Affiliation:1. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland;2. Department of Chemistry, University of Warmia and Mazury in Olsztyn, Plac Lodzki 4, 10-727 Olsztyn, Poland;3. Department of Systems Engineering, University of Warmia and Mazury in Olsztyn, Heweliusza 14, 10-718 Olsztyn, Poland
Abstract:The effect of high hydrostatic pressure (HHP) (450–550–650 MPa/5–10–15 min) on the carotenoid profile of purées obtained from commercial tomato varieties (Maliniak, Cerise, Black Prince and Lima) was investigated. The carotenoids profile was analysed using high performance liquid chromatography (HPLC-DAD). Photochemiluminescence (PCLACL), ferric-reducing antioxidant power (FRAP) and cyclic voltammetry (CV) assays were used to determine the antioxidant capacity of the lipophilic extracts. The results demonstrated that at certain processing conditions, HHP distinctly enhanced antioxidant capacity of the studied material. A significant reduction in lycopene and β-Carotene concentration was observed for all the HHP-treated purées. In contrast, lutein found in the purée extracts of Cerise and Black Prince tomatoes was slightly affected at 550 MPa/5 min and 650 MPa/5 min. Among all the carotenoids studied, lutein strongly and significantly affected PCLACL and CV parameters. In contrast, lycopene had a negligible effect on the examined parameters.
Keywords:Carotenes  cyclic voltammetry  ferric reducing antioxidant power  HPLC-DAD  lutein  photochemiluminescence  pressurisation  tomato processing
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