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Bioactive compounds and antioxidant activities of Brazilian hop (Humulus lupulus L.) extracts
Authors:Aline da Rosa Almeida  Matheus Vinícius de Oliveira Brisola Maciel  Michelle Heck Machado  Giovana Carolina Bazzo  Rafael Dutra de Armas  Vinicius Bittencourt Vitorino  Luciano Vitali  Jane Mara Block  Pedro Luiz Manique Barreto
Affiliation:1. Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, 88034-102 Brazil;2. Department of Biomedicine, Catholic University Center of Santa Catarina, Joinville, SC, 89203-005 Brazil;3. Department of Chemistry, Federal University of Santa Catarina, Florianópolis, SC, 88040-970 Brazil
Abstract:Bioactive compounds from Brazilian hop (Humulus lupulus L.) cultivars were extracted by ultrasound and their phenolic profile compared with commercial hop from the USA. The most effective extraction conditions (solution of ethanol 49%, at 52 °C and a solid/liquid ratio of 1 g per 34 mL) for the total phenolic compounds (TPC) were determined using a Central Composite Rotatable Design. The Brazilian hop showed higher content of TPC (33.93 ± 0.67 mg GAE g−1), total flavonoids (54.47 ± 0.10 mg QE g−1) and higher antioxidant activity (ABTS: EC50 21.29 ± 1.36 μL mL−1; DPPH: EC50 3.91 ± 0.17 μL mL−1) when compared with the USA hop. The main phenolic compounds present in the extracts were the flavonoids isoquercitrin and quercetin. The antioxidant properties of the Brazilian hop extract had not been reported yet in the literature for this raw material, thus showing potential to be incorporated in polymeric films used as active packaging.
Keywords:Antioxidant activity  Brazilian hop  flavonoids  phenolic compounds
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