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Effects of carboxymethylation,hydroxypropylation and dual-enzyme hydrolysis combination with heating on in vitro hypoglycaemic properties of coconut cake dietary fibres
Authors:Yajun Zheng  Panqi Shi  Yan Li  Zhuang Yongliang  Xian Wang  Le Liu
Affiliation:1. College of Food Science, Shanxi Normal University, Linfen, 041004 China;2. College of Food Science, Shanxi Normal University, Linfen, 041004 China

The two authors contributed equally to this work, and they are co-first authors.

Contribution: Data curation, Formal analysis, ?Investigation;3. College of Food Science, Shanxi Normal University, Linfen, 041004 China

Contribution: Data curation, Formal analysis, Writing - review & editing;4. Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan, 650500 China

Contribution: Validation, Writing - review & editing;5. College of Food Science, Shanxi Normal University, Linfen, 041004 China

Contribution: ?Investigation, Software;6. College of Food Science, Shanxi Normal University, Linfen, 041004 China

Contribution: Data curation, Methodology

Abstract:Effects of carboxymethylation, hydroxypropylation and dual-enzyme hydrolysis combined with heating on in vitro hypoglycaemic properties of coconut cake dietary fibre (CCDF) were studied. Results showed that all the three modification methods could effectively improve (P < 0.05) the glucose-adsorption ability (GAA), glucose dialysis retardation index (GDRI), α-glucosidase and α-amylase inhibition activity of CCDF. The highest GAA (4.45–4.93 mm  g?1), GDRI (85.09–86.94% mm  g?1) and α-glucosidase inhibition activity (16.39–19.37%) were found on CCDFs modified by hydroxypropylation and carboxymethylation, attributed to the increased soluble dietary fibre content, viscosity and water retention and swelling capacity. Moreover, CCDF treated by enzymatic hydrolysis combined with heating demonstrated the highest α-amylase inhibition activity (53.95%), attributed to the high specific surface area, more porous surface structure and formation of fibre–amylase complex proved by fluorescence spectroscopy. These results suggest that the modified CCDFs could be used as low-calorie functional ingredients in food or other industries.
Keywords:Carboxymethylation  coconut cake dietary fibre  dual-enzyme hydrolysis combined with heating  hydroxypropylation  hypoglycaemic property
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