Effects of protein content and rumen-undegradable to rumen-degradable protein ratio in finely ground calf starters on growth performance,ruminal and blood parameters,and urinary purine derivatives

This study investigated the effects of feeding finely ground starter diets containing either 18 or 22% crude protein (CP) content dry matter (DM) basis] and high or low ratios of rumen-undegradable protein to rumen-degradable protein (RUP:RDP) on growth performance, nutrient digestibility, ruminal fermentation, blood metabolites, and urinary purine derivatives in dairy calves. A total of 48 three-day-old female Holstein dairy calves with 40.2 ± 2.5 kg of initial body weight (BW) were randomly assigned in a complete randomized block design to a 2 × 2 factorial arrangement of treatments (12 calves/treatment). Treatments were as follows: (1) finely ground starter diet (mean particle size = 0.69 mm) with 18% CP and low RUP:RDP ratio low ratio (LR) = 26:74; 18CP-LR]; (2) finely ground starter diet with 18% CP and high RUP:RDP ratio high ratio (HR) = 35:65; 18CP-HR]; (3) finely ground starter diet with 22% CP and low RUP:RDP ratio (22CP-LR); (4) finely ground starter diet with 22% CP and high RUP:RDP ratio (22CP-HR) on DM bases. Blocking was based on the day of treatment assignment, and treatments were randomly assigned within each block. Calves received 4 L of milk daily from d 3 to 10, 7 L/d from d 11 to 40, 4 L/d from d 41 to 49, and 2.5 L/d from d 50 to 53, and then all calves were weaned but remained in the experiment until d 83 of age. The results showed that overall average daily gain (ADG), weaning BW, and feed efficiency (FE) were greater in 22% CP treatments than in 18% CP. Increasing the starter CP content from 18 to 22% of DM did not influence overall starter feed intake, milk intake, total dry matter intake (DMI), postweaning ADG, and FE of calves. No effect of RUP:RDP ratio was observed for starter feed intake, milk intake, total DMI, preweaning ADG, FE, and grams of CP per megacalorie of metabolizable energy. The RUP intake and postweaning ADG were greater for calves fed the HR diets than for those fed the LR diets. The digestibility of neutral detergent fiber was greater, and the digestibility of OM tended to be greater, and the ruminal concentrations of total short-chain fatty acids (SCFA), acetate proportion, and acetate-to-propionate ratio were greater in 22% CP than in 18% CP. A 2-way interaction between starter protein content and time was observed for total ruminal SCFA, acetate proportion, and acetate-to-propionate ratio, indicating that starter CP concentration had more effect on ruminal parameters. Preweaning urinary purine derivatives, preweaning microbial protein synthesis, and postweaning urinary nitrogen were greater for calves fed the 22CP diets than for those fed the 18CP diets but were not affected by the different RUP:RDP ratios. The concentrations of blood glucose and insulin were greater in 22% CP than in 18% CP diets. The blood insulin concentration was greater when calves received the HR diets compared with the LR diets. Therefore, we conclude that greater starter protein content can have beneficial effects on growth performance, probably through increased microbial protein synthesized and preweaning blood insulin concentration; however, a greater RUP:RDP ratio showed marginal effects on growth performance during the postweaning period.