Bacteriophage has beneficial effects in a murine model of Klebsiella pneumoniae mastitis |
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Authors: | Wenpeng Zhao Yuxiang Shi Gang Liu Jingyue Yang Bing Yi Yongxia Liu John P Kastelic Bo Han Jian Gao |
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Affiliation: | 1. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China;2. College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, Hebei 056038, P.R. China;3. College of Veterinary Medicine, Shandong Agricultural University, Tai`an 271018, China;4. Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada, T2N 4N1 |
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Abstract: | Bovine mastitis caused by Klebsiella pneumoniae is usually treated with antibiotics, thereby potentially increasing antimicrobial resistance. The objective of this study was to evaluate efficacy of a bacteriophage, isolated from dairy farm wastewater, as a treatment for a murine model of K. pneumoniae mastitis. A lytic bacteriophage CM8–1 was isolated, morphological and biological characteristics were assessed with transmission electron microscopy and double-layer plate, and its genome was sequenced and analyzed. Furthermore, effectiveness of this bacteriophage for treatment of a murine model of K. pneumoniae mastitis was evaluated based on the following mammary gland characteristics: morphological changes; number of K. pneumoniae; and mRNA and protein expression of pro-inflammatory factors TNF-α, IL-1β, IL-6, and IL-8. Bacteriophage CM8–1 had an incubation period of 30 min and a burst time of 20 min. Its viability and adsorption were stable at 30 to 50°C, but decreased significantly at >60°C, with no significant change in viability or infectivity at pH 6 to 10. In a murine model of K. pneumoniae mastitis, injecting bacteriophage CM8–1 into the mammary gland 2 h after inoculation with K. pneumoniae resulted in reductions in bacterial counts in the murine mammary gland, improvements in mammary gland tissue morphology, and reductions in mRNA and protein expression of pro-inflammatory factors. Bacteriophage CM8–1 had stable biological characteristics and suppressed K. pneumoniae mastitis when injected into the mammary gland 2 h latera in mice bacterial inoculation. |
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Keywords: | bovine mastitis bacteriophage antibiotic alternatives murine mastitis model |
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