微波预处理提取泽泻中三萜总组分的研究

将微波预处理技术应用在泽泻三萜总组分的提取中,采用单因素试验法,考察了物料颗粒大小、预处理剂浓度及用量、预处理时间、后续提取时间和后续提取溶剂浓度对提取率的影响,并运用正交试验对预处理工艺参数进行了优化。确定提取工艺参数为:泽泻取中颗粒(40目),预处理剂为1.9倍物料量(ml/g)的50%乙醇,辐射时间180s,85%乙醇常规回流提取2次,每次30min,提取率可达到1.784%。

Extraction of Total Triterpenoids from the Rhizoma of Alisma orientalis (Sam.) Juzep by Microwave Pretreatment

The extraction of total triterpenoids(TT) from the rhizoma of Alisma orientalis (Sam.) Juzep by microwave pretreatment technique has been explored. Its parameters, such as particle size of the rhizoma, concentration and quantity of pretreatment solvent, radiation time, extract time after microwave pretreatment and concentration of solvent used after micro- wave pretreatment,were analyzed by single-factor tests. The optimal pretreatment condition was obtained by orthogonal test. The suitable extraction conditions indicate that the 40-mesh powder of the rhizoma is extracted two times by reflux with 85% ethanol for 30min after pretreated by 50% ethanol used for 1.9ml per gram rhizoma powder and radiated 180s in microwave. The yield of TT is 1.784%.