Solid extraction of caffeine and theophylline from green tea by molecular imprinted polymers |
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Authors: | Dexian?Wang Seung?Pyo?Hong Email author" target="_blank">Kyung?Ho?RowEmail author |
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Affiliation: | (1) Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, 402-751 Nam-Ku, Incheon, Korea |
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Abstract: | This paper involves a feasibility study on using molecular imprinted polymers as the sorbent materials in solid phase extraction
for caffeine and theophylline from green tea. Two kinds of MIPs, with caffeine-theophylline mixture and pentoxifylline-theophylline
mixture as the templates respectively, MAA as the monomer, EDMA as the crosslinker and ATBN as the initiator, were applied
to this purpose. Mixture solution of caffeine and theophylline (1 Μg/ ml in acetonitrile) was applied to the solid extraction
cartridges following a load, wash and elute procedure with acetonitrile, methanol, methanol-acetic acid (90/10, v/v) as the
solvents, respectively. This solid phase extraction protocol was applied for extraction of caffeine and theophylline from
green tea. Comparison between the results obtained with the MIPs cartridges and a traditional C18 reversed-phase cartridge was made. It showed that the MIP-based sorbent on the solid phase extraction was comparable with
that of C18 material. HPLC analysis using a C18 column (5 Μm, 250× 4.6 mm from Rstech corporation), methanol: water (60 :40, v/v) as the mobile phase at a flow rate of 0.6
ml/min was applied for the quantitative determination. |
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Keywords: | Molecular Imprinted Polymers Solid Phase Extraction Caffeine Theophylline |
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