Size-dependant cellular uptake of dendritic polyglycerol |
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Authors: | Reichert Stephanie Welker Pia Calderón Marcelo Khandare Jayant Mangoldt Dorothea Licha Kai Kainthan Rajesh K Brooks Donald E Haag Rainer |
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Affiliation: | 1. Department of Biology, Chemistry, and Pharmacy, Freie Universit?t Berlin, Takustrasse 3, 14195 Berlin, Germany;2. Mivenion GmbH, Robert‐Koch‐Platz 4, 10115 Berlin, Germany;3. Department of Pathology and Laboratory Medicine, Centre for Blood Research, University of British Columbia, Vancouver, BC, V6T 2B5, Canada |
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Abstract: | To study the mechanism of cellular internalization, hyperbranched polyether derivatives consisting of amino-bearing hyperbranched polyglycerols (HPGs) of varied molecular mass and size range are designed and synthesized. HPGs were further fluorescently labelled by conjugating maleimido indocarbocyanine dye (ICC-mal). The conjugates are characterized by UV-vis spectroscopy, fluorescence profile, zeta potential, and dynamic light scattering. The uptake mechanism is studied by fluorescence-activated cell sorting (FACS) analysis, fluorescence spectroscopy, and confocal microscopy with human lung cancer cells A549, human epidermoid carcinoma cells A431, and human umbilical vein endothelial cells (HUVEC) cells. For the first time, the results suggest that the higher-molecular-weight HPGs (40-870 kDa) predominantly accumulate in the cytoplasm much better than their low-molecular-weight counterparts (2-20 kDa). The HPG nanocarriers discussed here have many biomedical implications, particularly for delivering drugs to the targeted site. |
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Keywords: | biomedical application dendrimer drug delivery polyglycerols nanocarriers |
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