Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman |
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Authors: | Aguiar Jaciana S Araújo Rosilma O Rodrigues Maria do Desterro Sena Kêsia X F R Batista André M Guerra Maria M P Oliveira Steno L Tavares Josean F Silva Marcelo S Nascimento Silene C da Silva Teresinha Gonçalves |
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Affiliation: | Antibiotics of Departament, Federal University of Pernambuco, 50670-901, Recife, PE, Brasil; E-Mails: jacianaaguiar@gmail.com (J.S.A.); rosilma23@hotmail.com (R.O.A.); mdrodrigues@yahoo.com.br (M.D.R.); kxfrs@bol.com.br (K.X.F.R.S.); silenen@yahoo.com.br (S.C.N.). |
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Abstract: | In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6β-Benzoyloxy-3α-(3,4,5- trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 μg/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95:5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95:5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis. |
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Keywords: | Erythroxylum caatingae antimicrobial activity cytotoxic activity hemolytic activity apoptosis |
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