| 栉孔扇贝血淋巴细胞HSP70表达变化的FCM法检测 |
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| 引用本文： | 曲凌云,孙修勤,相建海,洪旭光,张进兴,王亮,李继业.栉孔扇贝血淋巴细胞HSP70表达变化的FCM法检测[J].高技术通讯,2004,14(4):94-96. |
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| 作者姓名： | 曲凌云 孙修勤 相建海 洪旭光 张进兴 王亮 李继业 |
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| 作者单位： | 国家海洋局第一海洋研究所,青岛,266061;中国科学院海洋研究所,青岛,266071;中国科学院研究生院,北京,100039;国家海洋局第一海洋研究所,青岛,266061;中国科学院海洋研究所,青岛,266071 |
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| 基金项目： | 863计划 ( 2 0 0 2AA62 815 0 )、973规划 (G199912 0 0 5 )，国家海洋局青年基金 ( 2 0 0 3 10 3 )资助项目 |
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| 摘 要： | 建立了热激、冷激、细菌感染和水质恶化等逆境中栉孔扇贝血淋巴细胞HSP70表达变化的流式细胞术检测法。结果发现，该方法可灵敏检出上述条件下，血淋巴细胞中HSP70表达的增加。Duncan检验显示，热激条件下HSP70表达增加最为显著。
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| 关 键 词： | 栉孔扇贝 血淋巴细胞 HSP70 流式细胞术 |
Flow Cytometer Detection of HSP70 Expression in Haemocytes of Chlamys farreri |
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| Qu Lingyun,Sun Xiuqin,Xiang Jianhai,Hong Xuguang,Zhang Jinxing,Wang Liang,Li Jiye.Flow Cytometer Detection of HSP70 Expression in Haemocytes of Chlamys farreri[J].High Technology Letters,2004,14(4):94-96. |
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| Authors: | Qu Lingyun Sun Xiuqin Xiang Jianhai Hong Xuguang Zhang Jinxing Wang Liang Li Jiye |
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| Abstract: | Flow cytometry was used in this paper to evaluate HSP70 in haemocytes of Chlamys farreri, which had been undergone following stresses: heat, cold, infection and degradation. Results showed that HSP70 increased remarkably in the haemocytes and flow cytometry was a simple, agile means for this study. Duncan test showed that heat aroused the most remarkable increasing of HSP70. |
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| Keywords: | Chlamys farreri Haemocytes HSP70 Flow cytometry |
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