快速检测病原菌含量的简易微流控系统

针对现有水产养殖病原菌检测时效性差、自动化程度低的缺点,提出了一种快速检测水产养殖病原菌含量的简易微流控系统研究方法。该系统将微电极构造于微流控芯片通道底部并与数字阻抗测量电路连接,通过在磁珠表面包被水产病原菌抗体,利用外部高斯磁场控制诱导磁珠捕获水产病原菌,并将水产病原菌与磁珠结合体带至微电极阵列,通过在电极端构建基于阻抗测量的计数电路,从而有效检测水产病原菌含量。研究结果表明该系统能够有效测量水产病原菌的含量,检测时间由传统的平板计数法48 h减少至30 min,检测效率提高为原来的60多倍,检出限相比现有的微流控阻抗法缩小100倍。

Simple microfluidic system for rapid detection of aquatic pathogenic bacteria

To overcome the poor timeliness and the low automaticity of existing aquaculture pathogen detection, an integrated microfluidic system for rapid detection is proposed. The microelectrode is set on the bottom of the microfluidic channel and connected to a digital impedance measurement circuit in this system. The magnetic beads is encased by the aquatic pathogens antibody on its surface. The aquatic pathogens are captured by magnetic beads because of the induction and control of the external Gauss magnetic field. The combination of aquatic pathogens and beads is induced to the microelectrode array. The content of aquatic pathogens can be detected effectually by the counting circuit based on impedance measurement fixed at the electrode terminals. The research results show that the amount of aquatic pathogens can be detected effectively by this system. Moreover, the proposed system can reduce the detection time to one sixtieth compared with traditional laboratory test.