| 谷氨酰胺转胺酶高产菌株的选育 |
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| 引用本文： | 陈国娟,张春红,刘长江.谷氨酰胺转胺酶高产菌株的选育[J].食品科技,2006,31(2):15-17. |
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| 作者姓名： | 陈国娟 张春红 刘长江 |
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| 作者单位： | 沈阳农业大学食品学院,沈阳,110161 |
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| 摘 要： | 通过对谷氨酰胺转胺酶生产菌株灰轮丝链轮丝菌ZC03(酶活0.58U/mL)的孢子进行紫外线及He-Ne激光复合诱变,筛选获得一株性状稳定、产酶能力显著提高的突变株ZC60(酶活0.91U/mL),诱变条件为:紫外灯功率15W,距离30cm,照射时间60s,He-Ne激光波长632.8nm,功率1.5mW,距离30cm,照射时间为10min。
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| 关 键 词： | 谷氨酰胺转胺酶 灰轮丝链轮丝菌 诱变 筛选 |
| 文章编号： | 1005-9989(2006)02-0015-03 |
| 修稿时间： | 2005年8月28日 |
Screening of microbe strain for high-production of microbial transglutaminase |
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| CHEN Guo-juan,ZHANG Chun-hong,LIU Chang-jiang.Screening of microbe strain for high-production of microbial transglutaminase[J].Food Science and Technology,2006,31(2):15-17. |
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| Authors: | CHEN Guo-juan ZHANG Chun-hong LIU Chang-jiang |
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| Abstract: | Streptoverticillium griseoverticillatum-ZC03, which producing transglutaminase at the activity of 0.58U/mL, was mutated with ultraviolet and He-Ne laser. A stable and high-produced strains was obtained by fermentation screening, which producing microbial transglutaminase at the activity of 0.91U/mL. The mutation condition lies that the ultraviolet light strength is 15W, vertical distance is 30cm, and shining time is 60 seconds. The He-Ne laser strength is 1.5mW, vertical distance is 30cm, wave length is 632.8nm, and shining time is 10 minutes. |
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| Keywords: | transglutaminase streptoverticillium griseoverticillatum mutate screen |
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