Porous Poly(ε‐caprolactone) Nerve Guide Filled with Porous Gelatin Matrix for Nerve Tissue Engineering |
| |
| Authors: | Chiara Tonda‐Turo Chiara Audisio Sara Gnavi Valeria Chiono Piergiorgio Gentile Stefania Raimondo Stefano Geuna Isabelle Perroteau Gianluca Ciardelli |
| |
| Affiliation: | 1. Department of Mechanics, Politecnico di Torino Corso Duca degli Abruzzi 24, 10129 Turin, (Italy);2. Department of Animal and Human Biology, University of Turin Via Accademia Albertina 13, 10123 Turin, (Italy);3. Neuroscience Institute “Cavalieri Ottolenghi” (NICO) & Department of Clinical and Biological Sciences, University of Turin Regione Gonzole 10, 10043 Orbassano, (Italy) |
| |
| Abstract: | The preparation and characterization of PCL guides filled with a porous matrix of a freeze‐dried, genipin‐crosslinked gelatin sponge (GL/GP) is described. Porous guides are obtained by dip coating a rotating mandrel with a PCL/PEO blend solution followed by PEO solvent extraction. PCL/PEO 60/40 is selected as the optimal composition to obtain model porous membranes with suitable morphological properties to ensure nutrient permeation and moderate stiffness. GL/GP sponges show increased mechanical strength and water stability, compared to uncrosslinked GL sponges. GL/GP in the form of films, sponges and internal fillers support the in vitro adhesion and proliferation of Schwann like cells. |
| |
| Keywords: | |
|
|
