Liquefied Capsules Coated with Multilayered Polyelectrolyte Films for Cell Immobilization |
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Authors: | Nazua L Costa Praveen Sher João F Mano |
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Affiliation: | 1. 3B's Research Group – Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark, 4806‐909 Taipas, Guimar?es, (Portugal);2. IBB (Institute for Biotechnology and Bioengineering), PT Government Associated Laboratory Guimar?es, (Portugal) |
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Abstract: | Natural‐derived polymers are used to coat liquid‐core capsules layer by layer to encapsulate cells. Human osteoblast‐like cells (SaOs‐2) are encapsulated in such spherical devices using a three‐step methodology: i) ionotropic gelation to produce alginate beads encapsulating the cells; ii) layer‐by‐layer coating using water‐soluble chitosan and alginate; and iii) core liquefaction. Cells remain viable for 3 d after the encapsulation procedure, suggesting that the developed capsules possess a semipermeable, nanostructured coating. All of the capsules exhibit a spherical shape, smooth surface and liquid‐core characteristics. All of the processes are conducted under mild conditions and physiological pH. We consider that the methodology employed in the development of the capsules obtained from natural‐based biomaterials has potential to find applicability in the development of scaffolds or cell carriers in tissue engineering and regenerative medicine. |
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