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黑木耳多糖的酶法提取、纯化及性质研究
引用本文:韩春然,唐娟,马永强.黑木耳多糖的酶法提取、纯化及性质研究[J].食品科学,2007,28(2):53-55.
作者姓名:韩春然  唐娟  马永强
作者单位:哈尔滨商业大学食品工程学院; 哈尔滨商业大学食品工程学院 黑龙江哈尔滨150076; 黑龙江哈尔滨150076;
基金项目:黑龙江省教育厅科学技术研究项目
摘    要:本文以双酶法提取黑木耳粗多糖,利用DEAE-Cellulose 52和Sepharose CL-4B柱层析对其进行纯化,以Sephadex G-100凝胶柱层析测定了多糖的分子量。结果表明黑木耳多糖含有两个主要组分APE Ⅰ和APE Ⅱ,分子量分别为31.7×104和18.3×104,均为非淀粉类多糖,不含单糖、蛋白质、核酸及多酚类物质。

关 键 词:黑木耳    多糖    层析    纯化    分子量  
文章编号:1002-6630(2007)02-0053-04
修稿时间:2005-11-16

Study on Isolation, Purification and Characteristics of Polysaccharides from Auricularia auricula
HAN Chun-ran,TANG Juan,MA Yong-qiang.Study on Isolation, Purification and Characteristics of Polysaccharides from Auricularia auricula[J].Food Science,2007,28(2):53-55.
Authors:HAN Chun-ran  TANG Juan  MA Yong-qiang
Affiliation:College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
Abstract:The Auricularia auricular polysaccharides were extracted by proteinase and celluloase, purified by DEAE- Cellulose 52 and Sepharose CL-4B chromatography.The molecular weight was determined by Sephadex G -100 chromatograph. The results showed that there are two main fractions in Auricularia auricular polysaccharides with the molecular weight of 31.7×104 and 18.3×104, respectively, which are non-starch polysaccharides, free of simple sugar, protein, nuclear acid and phenols.
Keywords:Auricularia auricular  polysaccharides  chromatography  purify  molecular weight
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