己酸菌W_1的分离特性及产酸条件的研究

已酸菌W_1是从宜宾五粮液酒厂的500年老窖泥中分离得到的高产已酸的菌株,经分类鉴定,该菌与克氏梭菌(Clostridium kluyverii)的特性很不相同,因此,无法划入克氏梭菌种内,暂定(Clostridium sp.W_1)。 经对W_1菌产酸条件的研究,该菌属于耐气性厌氧菌,用简单的液体合成培养基深层培养,就可满足生长。该菌生长的适宜温度为34℃,适宜的产酸的pH范围为6.5～7.5,乙醇和乙酸钠是该菌生长和产已酸的主要基质。

Isolation Characterization and the Culture Coditions of Clostridium Sp. W_1

The caproic acid-producing bacterium W, was isolated from Sludge of 500-years old fermentpits in Yibin wuliangye distillery. It produces high level of caproic acid. According to classificatory identification, the stran was different from Clostridium kluyverii. Thus we debignated it as Clostridium sp.W1.This oxygen-tolerant anaerobe grows well in deep culture of relatively simple synthetic media with the optimal temperature and pH being 3412 and 6.5-7.5 respectively The main substrates for growth and caproic acid production are ethaonl and sodium ethanaic.