| 一株高效反硝化聚磷菌的筛选及脱氮除磷效能 |
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| 引用本文： | 马放,杨菲菲,张倩,李昂,姜欣欣,张晓昕. 一株高效反硝化聚磷菌的筛选及脱氮除磷效能[J]. 哈尔滨工业大学学报, 2011, 0(12): 42-47 |
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| 作者姓名： | 马放 杨菲菲 张倩 李昂 姜欣欣 张晓昕 |
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| 作者单位： | 哈尔滨工业大学城市水资源与水环境国家重点实验室;辽宁大学环境学院;武汉大学土木建筑工程学院 |
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| 基金项目： | 国家自然科学基金资助项目(51178139,51108120);国家水专项资助项目(2008ZX07202-005);第四批中国博士后科学基金特别资助项目(201104430);第46批中国博士后科学基金项目(20090460901) |
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| 摘 要： | 高效的反硝化聚磷菌的分离及脱氮除磷效能的研究对于反硝化聚磷机理及脱氮除磷工艺的创新具有重要意义.采用专性培养基,从稳定运行的A/O/A SBR反应器中分离得到16株反硝化聚磷菌.通过poly-P染色试验、吸磷试验、硝酸盐还原产气及脱氮效能试验相结合的方法,筛选得到一株高效的反硝化聚磷菌Q-hrb05.实验结果表明：经1...
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| 关 键 词： | 生物除磷 反硝化聚磷菌 分离鉴定 系统发育 除磷率 聚-β-羟丁酸 |
Isolation and identification of denitrifying polyphosphate-accumulating organisms and its efficiency of nitrogen and phosphorus removal |
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| MA Fang,YANG Fei-fei,ZHANG Qian,LI Ang,JIANG Xin-xin,ZHANG Xiao-xin. Isolation and identification of denitrifying polyphosphate-accumulating organisms and its efficiency of nitrogen and phosphorus removal[J]. Journal of Harbin Institute of Technology, 2011, 0(12): 42-47 |
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| Authors: | MA Fang YANG Fei-fei ZHANG Qian LI Ang JIANG Xin-xin ZHANG Xiao-xin |
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| Affiliation: | 1(1.State Key Lab of Urban Water Resource and Environment,Harbin Institute of Technology,150090 Harbin, China;2.School of Environmental Science,Liaoning University,110036 Shenyang, China;3.School of Civil Engineering,Wuhan University,430071 Wuhan,China) |
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| Abstract: | In this paper,16 denitrifying phosphate-accumulating organisms(DNPAOs) were isolated in the special medium from the anaerobic/aerobic/anoxic SBR reactor,and the most efficient DNPAOs called Q-hrb05,was screened by a phosphate uptake experiment,where nitrates were denitrified to gas and through inspection of the poly-P.Strain Q-hrb05 was identified by 16S rDNA gene analysis,and the accession number of 16 S rDNA gene sequence of strain Q-hrb05 in GenBank was GU214826.The result showed that the strain Q-hrb05 belonged to Bacillus sp,of which the homology could reach to 99%,that also corresponded to the result of biochemical features.The average phosphorus uptake rate of strain Q-hrb05 was at 85% within 12 hours,and it had the denitrification capacity in the aerobic condition.It could be found that the phosphorus uptake rate was related to the content change of PHB.Under anaerobic condition,the maximal P-release rate was 66%,which could reach to 12% of the dry cell weight.The P-uptake rate was 92% with the decreasing of PHB content. |
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| Keywords: | biological phosphorus removal denitrifying polyphosphate-accumulating organisms isolation and identification phylogenetic analysis phosphorus release rate Poly-β-phydroxybutyrate |
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