Fluorescence-based assay formats and signal amplification strategies for DNA microarray analysis |
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Authors: | Hyun Gyu Park Jae Yang Song Min Hwan Kim |
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Affiliation: | a Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea b RapleGene.Inc, 65 Bokjung-dong, Sujung-gu, Sungnam, Kyunggi 461-701, Korea |
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Abstract: | DNA microarrays are powerful tools for the high throughput analysis of nucleic acids due to their parallel detection capabilities. To realize the apparent power of DNA microarray, an efficient assay format is essential and a variety of assay formats have been developed for nucleic acid detection on microarrays. Many of them employ fluorescence-based methods because fluorescence detection is straightforward and easy to implement. Herein we broadly review fluorescence-based assay formats with a focus on PCR-associated target preparation. For the heterogeneous assay of nucleic acid on DNA microarrays, sensitivity is one of the most important factors and special emphasis has been given to recently developed signal amplification strategies aimed at achieving high sensitivity. |
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Keywords: | Fluorescence DNA microarray DNA analysis Signal amplification Target preparation |
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