| HCV e2-3pcx融合基因原核表达载体的构建及蛋白的初步表达 |
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| 引用本文： | 李凤梅,刘均洪,任大明. HCV e2-3pcx融合基因原核表达载体的构建及蛋白的初步表达[J]. 青岛科技大学学报(自然科学版), 2007, 28(5): 383-385 |
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| 作者姓名： | 李凤梅 刘均洪 任大明 |
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| 作者单位： | 青岛科技大学,化工学院,山东,青岛,266042;复旦大学,遗传学研究所遗传工程国家重点实验室,上海,200433 |
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| 摘 要： | 构建了丙型肝炎病毒e2-3pcx融合基因的原核表达载体pET28b(+)-e2-3pcx,并转化到大肠杆菌BL21(DE3)菌株中。IPTG诱导融合蛋白高效表达。经SDS-PAGE电泳分析,结果表明在43 ku处有一条蛋白质特异条带,与预期的目的产物蛋白带大小一致。
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| 关 键 词： | e2基因 3pcx基因 原核表达 |
| 文章编号： | 1672-6987（2007）05-0383-03 |
| 收稿时间： | 2006-11-03 |
| 修稿时间： | 2006-11-03 |
Preliminary Construction and Expression of HCV e2-3pcx Fusion-gene Vector in E. coli |
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| LI Feng-mei,LIU Jun-hong,REN Da-ming. Preliminary Construction and Expression of HCV e2-3pcx Fusion-gene Vector in E. coli[J]. Journal of Qingdao University of Science and Technology:Natutral Science Edition, 2007, 28(5): 383-385 |
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| Authors: | LI Feng-mei LIU Jun-hong REN Da-ming |
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| Affiliation: | 1.College of Chemical Engineering;Qingdao University of Science and Technology;Qingdao 266042;China;2.State Key Laboratory of Genetic Engineering;Institute of Genetics;Fudan University;Shanghai 200433;China |
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| Abstract: | Prokaryotic expression vector for e2-3pcx fusion-gene of Hepatitis C Virus(HCV) was constructed into pET28b(+)-e2-3pcx vector,and was transformed into BL21(DE3).Upon induction by IPTG,e2-3pcx fusion-gene was expressed as a major protein product in the total cellular protein of the recombinant vector compared with all the controls.SDS-PAGE was used to analyze the expression of the fusion protein.The result showed that there was a specific band at about 43 ku in size,which was identical with the expected molecular weight of the fusion protein. |
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| Keywords: | e2 gene 3pcx gene prokaryotic expression |
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