细胞谷胱甘肽含量与放射增敏研究

谷胱甘肽（ＧＳＨ）是一种内源性放射防护物质，在正常状况下大部分以还原型存在于细胞和组织中，本研究结果表明，通过外源性化学修饰剂丁胱亚磺酰亚胺（ＢＳＯ），可用效地抑制细胞内ＧＳＨ的生物合成，从而使内源性ＧＳＨ含量下降，在１ｍｍｏｌ／ＬＢＳＯ作用后２４ｈ，可使原有ＧＳＨ含量从４．６７６８４×１０＾－１２ｍｍｏｌ／ｃｅｌｌ下降至０．５０９２×１０＾－１２ｍｍｏｌ／ｃｅｌｌ为对照组的１．０９％，ＢＳＯ的抑

THE STUDY ON CELL GLIJTATHIONE LEVELS AND RADIOSENSITIZATION

The role of intracellar glutathione (GSH) in radiation protection has been noticed in recent years. In this study, we found that the level of intracellar glutathione and radiosensitization had a certain relationship. It was recognized that the higher GSH level, the lower radiosensitization. The GSH level in V 79-379 A, Y 79. SPC-A-1. SGC-7901 and So-Rb 50 cells were showed in this experiments by Tietze method. The GSH level which was depleted by BSO was assessed. The radiosensitization was determined by the standard colony formation assay iN VITRO.The results showed that SER (Sensitization Enhancement Ratio) for V 79-379 A cell was increased with GSH depletion. The SER for BSO 0. 1. 0. 5 and 1. 0 mmol/Lwere 1. 16, 1. 34 and 1. 50, respectively. Same results for Y 79 cells were also observed that the SER for BSO 0. 01 and 0. 05 mmol/L were 1. 21 and 1. 36 respedively.On the other hand, the exposure time of BSO also influenced GSH level and SER.These results suggested that the level of glutathione in cell and radiosensitization has a close relationship.