Green synthesis of silver nanoparticles from purple acid phosphatase apoenzyme isolated from a new source Limonia acidissima |
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Authors: | Omkar Pawar Neelima Deshpande Sharda Dagade |
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Affiliation: | 1. Department of Chemistry, H.V. Desai College, Pune, India;2. Department of Microbiology, Abasaheb Garware College, Pune 411004, India;3. Department of Chemistry, Y.M. College, Bharati Vidyapeeth, Pune, India |
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Abstract: | Green synthesis of nanoparticles is regarded as a safe and non-toxic process whereas conventional synthesis using chemical methods produces toxic substance. This study provides a novel insight for enzymatic synthesis method of silver nanoparticles using purple acid phosphatase, isolated from Limonia acidissima (wood apple) as a new source and used in the synthesis of silver nanoparticles. Stable silver nanoparticles were produced by sonochemical method using apoenzyme as a stabilising and capping agent and were characterised by various physicochemical techniques like UV–Visible spectroscopy, Fourier-transform infrared, X-ray diffraction and transmission electron microscopy. X-ray study shows that nanoparticles are composed of silver and silver oxide. The synthesised nanoparticles exhibited excellent antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. |
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Keywords: | silver nanoparticles purple acid phosphatase Limonia acidissima green synthesis |
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