Purification and characterization of a 28 kDa cytosolic inhibitor of cholesteryl ester hydrolases in rat testis |
| |
Authors: | Denise S. Hines Siowfong Wee W. McLean Grogan |
| |
Affiliation: | (1) Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, 23298-0614 Richmond, Virginia;(2) Present address: Immunex Corp., 51 University St., 98101 Seattle, WA |
| |
Abstract: | A 28 kDa inhibitory protein was purified from ratestis cytosol by sequential 40–65% ammonium sulfate precipitation, cation exchange chromatography, anion exchange chromatography, and preparative SDS-polyacrylamide gel electrophoresis. The heat-stable, trypsin-labile protein exhibited nonenzymatic, concentration-dependent inhibition of testicular and pancreatic cholesteryl ester hydrolases at all stages of putification. Copurifying at each stage was a 26.5 kDa protein which comprised 25% of the mass of the two proteins. Polyclonal antibodies raised to either or both 28 kDa and 26.5 kDa proteins by direct injection of excised electrophoretic bands cross-reacted with both proteins on western blots, immunoprecipitated both proteins, and neutralized inhibitory activity. Amino acid compositions of the individual proteins electroeluted from SDS-polyacrylamide gels were different from those of other surface-active proteins of similar molecular weights. Both proteins exhibited identical pl of 4.8 on chromatofocusing columns and two-dimensional gel electrophoresis. Although the subcellular distribution of the 28 kDa protein is unknown, its testicular cytosolic concentration, calculated from the purified protein mass, was 8×10−9 mols/L, which probably underestimates the actual concentration by an order of magnitude. This is greater than the minimum concentration required forin vitro inhibition (10−9 mols/L), consistent with a physiological role for this protein. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|