| 麦秸秆的氢氧化钙预处理及酶解试验研究 |
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| 引用本文： | 程旺开,汤斌,张庆庆,翟光雯,陈中碧.麦秸秆的氢氧化钙预处理及酶解试验研究[J].纤维素科学与技术,2009,17(1):41-45,51. |
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| 作者姓名： | 程旺开 汤斌 张庆庆 翟光雯 陈中碧 |
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| 作者单位： | 安徽工程科技学院,生化工程系,安徽,芜湖,241000 |
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| 基金项目： | 安徽省高校自然科学基金重点项目 |
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| 摘 要： | 采用氢氧化钙对麦秸秆进行预处理,以酶解还原糖得率为目的,分别优化预处理及酶解条件。结果表明,氢氧化钙预处理麦秸秆的最佳条件是:Ca(OH)2添加量为0.06g/g(对秸秆),固液比为1:10,在120℃下反应时间为2h;最佳酶解条件是:温度50℃,pH4.8,纤维素酶17FPU/g(对秸秆),木聚糖酶160IU/g,在添加0.15g/g(对秸秆)Tween80条件下,酶解液中还原糖质量浓度为62.32g/L,酶解还原糖得率达85.23%。
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| 关 键 词： | 麦秸秆 氢氧化钙 酶解 |
Study of Calcium Hydroxide Pretreatment and the Enzymatic Saccharification of Wheat Straw |
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| CHENG Wang-kai,TANG Bin,ZHANG Qing-qing,ZHAI Guang-wen,CHEN Zhong-bi.Study of Calcium Hydroxide Pretreatment and the Enzymatic Saccharification of Wheat Straw[J].Journal of Cellulose Science and Technology,2009,17(1):41-45,51. |
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| Authors: | CHENG Wang-kai TANG Bin ZHANG Qing-qing ZHAI Guang-wen CHEN Zhong-bi |
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| Affiliation: | (Department of Biochemistry and Engineering, Anhui University of Technology and Science, Wuhu 241000, China) |
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| Abstract: | In order to make the maximum yield of reducing sugars from wheat straw, the conditions of calcium hydroxide pretreatment and enzymatic saccharification of wheat straw were investigated. The optimal pretreatment conditions were： loading calcium hydroxide 0.06 g and water 10 g in per gram substrate, heating at 120℃ for 2 h. The optimal enzymatic saccharification conditions were： 0.15 g of Tween 80, 17 FPU of cellulase and 160 IU of xylanase per gram of substrate at 50℃, pH 4.8. The density of reducing sugar was 62.32 g/L, and the yield of reducing sugar reached 85.23%. |
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| Keywords: | wheat straw calcium hydroxide enzymatic hydrolysis |
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