中国板党中硒多糖提取分离技术研究

本研究以富含硒元素的恩施药材--中国板党为原料,对党参含硒多糖的分离提取技术进行了初步研究。实验采用水浸提、乙醇醇析提取、Sevage脱蛋白、干燥等方法研究中国板党含硒多糖的提取工艺,并用蒽酮比色法和原子吸收法对多糖含量、硒含量分别进行了测验。结果表明:板党水溶性含硒多糖最佳提取条件为:提取2次、加料液比为1:20、提取时间2h、提取温度为95℃。水溶性多糖含量为5.50%,多糖硒的含量为0.259μg/g。

Study on the Extraction and Separation of Se-Polysaccharides in Codonopsis pilosula from Banqiao

This article used the medicinal material Codonopsis pilosula which was rich in selenium element as experimental material, preliminarily studied the extracting and separating technology of Se-polysaccharides, the experiments studied the extracting technology by the methods of water extracting precipitation with alcohol, sevage deproteinizing, preciptation, drying and the content of polysaccharides and Se were determined by anthrone colorimetry and atomic absorption spectrometry. The optimum parameters to extract soluble Se-polysaccarides in Codonopsis pilosula were as follows: the extraction times is twice, the ration between material and solvent is 1: 20, the time is 2h, the temperature is 95℃. The content of soluble Se-polysaccarides is 5.5%, content of Se is 0.259%.