不同的壁材对辅酶Q10纳米脂质体包埋效果的影响

辅酶Q10是一种膳食补充剂或营养品,在人体细胞呼吸链的电子传递中起重要作用,采用纳米胶囊技术制备辅酶Q10纳米脂质体可提高其生物利用率。本文以包封率、产率、透光率(T500nm)和贮存稳定性(包括产品T500nm的变化以及芯材辅酶Q10的保留率)为评定指标,选用乙醇注入-超声法制备了包埋效果和贮存稳定性都较好的辅酶Q10纳米脂质体。结果表明,以蛋黄磷脂作为主要壁材制得产品的包埋效果及贮存稳定性均优于大豆磷脂,适量胆固醇以及吐温80的添加可显著改善包埋效果,壁材的最佳配比为:磷脂:胆固醇:吐温80=25:4:18(W/W),在最佳配比下将辅酶Q10与磷脂比提高至20:25(W/W,相应载量为40%)可制得包封率及保留率均高于95%的产品。

Effects of Different Wall Materials on Microencapsulation Quality of Coenzyme Q10 Nanoliposomes

Coenzyme Q10 (CoQ10), as dietary supplement or nutraceutical, is essential for cell respiration and electron transfer,helping control the production of energy in cells. The bioavailability of CoQ10 might be improved when using nanocapsule technology to prepare CoQ10 nanoliposomes. In this study,ethanol injection-sonication method was selected to prepare stable CoQ10 nanoliposomes with better microencapsulation quality. The encapsulation efficiency (EE), encapsulation yield (EY) and storage stability(including the change of transmittance at 500nm (T500nm) and the retention rate of core material (RR))of CoQ10 liposomes with eggyolk phosphatidylcholine (PC) as leading wall material were found better than those of CoQ10 liposomes with soybean PC when the loading capacity was higher. When using cholesterol and Tween 80 as adjuvant wall material, the product quality was improved evidently. The optimal wall formulation is obtained as follows: PC:Cholesterol: Tween 80=25:4:18 (W/ W). Under the conditions of optimal wall material formulation, both EE and RR of core material of CoQ10 nanoliposomes are higher than 95% when the ratio of CoQ10 to PC is increased to 20:25 (W/W, corresponding loading capacity is about 40%.).