A novel process for scopolamine separation from Hindu Datura extracts by liquid–liquid extraction,macroporous resins,and crystallization |
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Authors: | Chuanxiang Fu Pengpeng Chen Ailing Hui Yue Zheng |
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Affiliation: | Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, P. R. China |
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Abstract: | ABSTRACTAn efficient separation method of scopolamine from Hindu Datura extracts was successfully developed by combining liquid–liquid extraction, macroporous resins, and crystallization. First, the extraction solution was successively performed with liquid–liquid extraction by acid water and carbon tetrachloride. Secondly, D151 resin was selected from six tested resins, and the separation parameters were optimized. Finally, scopolamine hydrobromide was obtained after crystallization at ?20°C overnight. Through one run of above combined treatments, the content of scopolamine increased from 3.02% (w/w) to 99.12% (w/w). In addition, the products were assessed by high-performance liquid chromatography (HPLC). |
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Keywords: | Scopolamine liquid–liquid extraction macroporous resins crystallization |
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